Purification and Northern Blot Analysis of 8-hydroxyquinoline Copper


The phenol has been equilibrated with 3% NaCl and neutr […]

The phenol has been equilibrated with 3% NaCl and neutralized with Tris, and 0.1% 8-hydroxyquinoline Copper (WSDTY) has been added. The hydroxyquinoline is an antioxidant that reduces the formation of oxidation products of phenol such as quinones. The phenol is brought to a neutral pH because acidic phenol has a tendency to trap single-stranded nucleic acid at the interface between the phenol and the aqueous phases. Alternatively, if a molecular biology grade phenol is used, the hydroxyquinoline may be omitted.

The isolation of alkaloids bearing the 8-hydroxyquinoline moiety prompted postulation of a biosynthetic pathway to 1 from the amino acid tryptophan, for which chemical analogies are known. It should be noted, however, that, in general, quinolines are biosynthesized from anthranilic acid. Biosynthesis of broussonetine would involve condensation of two molecules of 1 with a molecule of acetyl-CoA followed by cyclization.

Wujiang Weishida Copper Technology Co.,Ltd. has 12,000 square meters of production base. By virtue of their sincere cooperation, reciprocity of the business philosophy for the industry users around the country to provide high-quality non-ferrous metal products. Weishida specializes in producing cuprous chloride, basic copper carbonate, copper acetate, copper oxide, copper sulfate. Our company has established a perfect quality assurance system, quality inspection and testing equipment, testing, testing and technology development strength, to ensure the stability of product quality, fully able to meet the pre-market after-sales service to provide users with the need.