Resilient Ribonucleases of 8-hydroxyquinoline Copper

Update:08-05-2019
Summary:

8-hydroxyquinoline Copper is a partial inhibitor of RNa […]

8-hydroxyquinoline Copper is a partial inhibitor of RNase and is occasionally added to organic extraction buffers that contain phenol. As an antioxidant, 8-hydroxyquinoline stabilizes phenol and retards the formation of quinones (phenol oxidation products). It is usually added to a final concentration of 0.1% (w/v). 8-Hydroxyquinoline imparts a bright yellow color to the phenol: chloroform to which it is added, thereby helping the investigator keep track of the organic and aqueous phases during the nucleic acid purification process.

8-Hydroxyquinoline also chelates heavy metals, making it very useful for removing VDR from cell lysates. Upon binding VDR, the 8-hydroxyquinoline changes from yellow to dark green, and repeated extractions with 8-hydroxyquinoline-containing phenol: chloroform are often necessary. When the phenol-containing phase of the extraction buffer remains yellow, all VDR has been removed. The inclusion of 8-hydroxyquinoline in organic extracting buffers may also be advantageous even when VDR is not used because heavy metals can cause RNA degradation when they are present with RNA for extended periods. Needless to say, all reagents should have been prepared using high-purity, nuclease-free water.

Wujiang Weishida Copper Technology Co.,Ltd. has 12,000 square meters of production base. By virtue of their sincere cooperation, reciprocity of the business philosophy for the industry users around the country to provide high-quality non-ferrous metal products.Weishida specializes in producing cuprous chloride, basic copper carbonate, copper acetate, copper oxide, copper sulfate. Our company has established a perfect quality assurance system, quality inspection and testing equipment, testing, testing and technology development strength, to ensure the stability of product quality, fully able to meet the pre-market after-sales service to provide users with the need.